Shanghai Medicilon Inc.

  Multidrug resistance is a serious threat to public health. Proton motive force-driven antiporters from the major facilitator superfamily (MFS) constitute a major group of multidrug-resistance transporters. Currently, no reports on crystal structures of MFS antiporters in complex with their substrates exist.  The E. coli  MdfA transporter is a well-studied model system for biochemical analyses of multidrug-resistance MFS antiporters. Here, we report three crystal structures of MdfA-ligand complexes at resolutions up to 2.0 ?, all in the inward-facing conformation. The substrate-binding site sits proximal to the conserved acidic residue, D34. Our mutagenesis studies support the structural observations of the substrate-binding mode and the notion that D34 responds to substrate binding by adjusting its protonation status. Taken together, our data unveil the substrate-binding mode of MFS antiporters and suggest a mechanism of transport via this group of transporters.

  Though opiate drug use has been much maligned over the past several years, the need for medically relevant painkillers has not waned. Scientists have been continually on the hunt for improved production methods of opiate compounds, as extraction from poppy sap is inefficient and time-consuming.     Now, a team of Japanese researchers from Ishikawa Prefectural University and Kyoto University has modified several genes from  Escherichia coli  to produce large quantities of the morphine precursor thebaine, which can be changed to make painkilling drug compounds. Moreover, the investigators found that their engineered  E. coli  produced 300 times more thebaine compared to a recently developed method involving yeast, in addition to having a much lower risk of unregulated production.     “Morphine has a complex molecular structure; because of this, the production of morphine and similar painkillers is expensive and time-consuming,” explained co-author Fumihiko Sato, Ph.D., professor in the

  E. coli  Expression System Services   E. coli  expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems,  E. coli  is a well-established host with the advantages of short culturing time, easy genetic manipulation and low cost media.   Medicilon researchers established a well-developed E. coli expression and purification services platform, which offers the   expression and purification services  of a variety of  recombinant proteins , with a fast turn-around time and competitive pricing. For more protein expression system,please click on  protein expression .   Service： Generation of Recombinant Plasmid Expression Test in Host Soluble Expression Test Small Scale Expression and Purification (2L Cell Culture) Scale Up Expression and Purification Steps Items Timeline Comments 1

  The prokaryotic expression system (E. coli expression system) is the earliest developed and most widely used classic expression system in gene expression technology. In recent decades, the   E. coli expression system   has also been continuously developed and improved, and has been widely used by scientific research and industrial users for various   Recombinant protein expression . Compared with other expression systems, it has the characteristics of high expression level of target gene, short culture period, strong anti-pollution ability and relatively low cost. Medicilon researchers have established a mature E. coli expression and  purification service  platform to provide expression and  purification services  including various recombinant proteins and their complexes in E. coli. Prokaryotic expression system (E. coli expression system) service items:    ❖ Recombinant plasmid construction;    ❖ Recombinant expression test;    ❖ Soluble expression condition test;    ❖ Small-scale

  Principle of Escherichia coli Expression System The  Escherichia coli expression system  is an expression system that efficiently expresses genes encoding protective antigens of pathogenic microorganisms in Escherichia coli through DNA recombination technology. It is currently the most commonly used system for recombinant protein expression. The E. coli expression system has been studied earlier and has many advantages such as clear background, simple operation, and high expression. The expression level is significantly higher than that of mammalian expression systems, but it also has corresponding shortcomings. For example, the E. coli system cannot secrete the expressed protein outside the cell, the ability to form disulfide bonds is limited, and complex modifications of protein expression (such as Glycosylation, etc.). Principle of Baculovirus Expression System Insect baculovirus expression vector system  is a recombinant protein production system that uses recombinant insect bacu

  Introduction In vitro  recombinant protein production  is a protein obtained by applying recombinant DNA or recombinant RNA technology. Catalog The use of in vitro protein expression technology to extensively produce various types of recombinant proteins, which are used in the fields of medicine, food, daily chemical and other fields. The following briefly introduces the main expression systems of the main recombinant proteins. Escherichia coli expression system Escherichia coli  has a clear genetic background, low cost, multiple vectors and hosts to choose from. It is not only the most commonly used expression system, but also the most economical protein expression system. The expression system is optimized, such as the compatibility test of the expression vector and the expression strain. , The optimization of induced expression conditions, renaturation, etc., can greatly solve the difficult problems of protein expression and insoluble protein expression. Suitable for proteins that

Recently, Huizhou Jizhong Biological Technology’s innovative drug local anesthetic injection - a new long-acting postoperative analgesic successfully obtained FDA (US Food and Drug Administration) new drug clinical trial approval! The new long-acting postoperative analgesic was completed by Shanghai Medicilon in its full set of  preclinical safety evaluation research ,  preclinical pharmacology ,  pharmacokinetics  and other projects. Anesthetics include general anesthetics, local anesthetics, narcotic analgesics, and muscle relaxants. Among them, local anesthetics are a class of drugs that are applied locally around nerve endings or nerve trunks, which can temporarily, completely and reversibly block the generation and conduction of nerve impulses, and temporarily disappear local pain sensation under conscious conditions. . Because local anesthetics are easy to use and have no damaging effects on various tissues, they have been widely recognized by clinicians. Currently, the commonly