Shanghai Medicilon Inc.

  Principle of Escherichia coli Expression System The  Escherichia coli expression system  is an expression system that efficiently expresses genes encoding protective antigens of pathogenic microorganisms in Escherichia coli through DNA recombination technology. It is currently the most commonly used system for recombinant protein expression. The E. coli expression system has been studied earlier and has many advantages such as clear background, simple operation, and high expression. The expression level is significantly higher than that of mammalian expression systems, but it also has corresponding shortcomings. For example, the E. coli system cannot secrete the expressed protein outside the cell, the ability to form disulfide bonds is limited, and complex modifications of protein expression (such as Glycosylation, etc.). Principle of Baculovirus Expression System Insect baculovirus expression vector system  is a recombinant protein production system that uses reco...

  Introduction In vitro  recombinant protein production  is a protein obtained by applying recombinant DNA or recombinant RNA technology. Catalog The use of in vitro protein expression technology to extensively produce various types of recombinant proteins, which are used in the fields of medicine, food, daily chemical and other fields. The following briefly introduces the main expression systems of the main recombinant proteins. Escherichia coli expression system Escherichia coli  has a clear genetic background, low cost, multiple vectors and hosts to choose from. It is not only the most commonly used expression system, but also the most economical protein expression system. The expression system is optimized, such as the compatibility test of the expression vector and the expression strain. , The optimization of induced expression conditions, renaturation, etc., can greatly solve the difficult problems of protein expression and insoluble protein expression. Suitabl...