Shanghai Medicilon Inc.

  What is a Primer? A primer is a short synthetic oligonucleotide which is used in many molecular techniques from  PCR  to DNA sequencing. These primers are designed to have a sequence which is the reverse complement of a region of template or target DNA to which we wish the primer to anneal. PCR Protocols 1. Mix the DNA polymerase, dNTP, DNA templates and DNA primers in a clean PCR tube. Then put the tube in PCR machine and begin setting 2. Pre-Denaturation: 95°C for 10min 3. Denaturation: 95°C for 30s 4. Annealing: 55°C±10°C (The exact temperature is determined by the length and GC percentage of your primers. You could find an answer in the user manual attached with your purchased primers) 5. Elongation: 72°C (The exact temperature is determined by the DNA polymerase you will use. You could find an answer in the user manual attached with the DNA polymerase you have purchased) 6. Cycle: Set the PCR machine to cycle from step 2 to step 4 between 20 and 30 times 7. Final E...