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The Role of the Ames Test in Predicting the Genotoxicity of Impurity Compounds in Drugs

  Many impurities in drugs can produce toxic and side effects, which is a crucial factor affecting the   safety and effectiveness of drugs , especially genotoxic impurities that can cause serious harm to human health with minimal exposure. Therefore, to ensure API quality and preparations, Genotoxic impurities are a class of impurities that must be paid attention to in the drug synthesis process. Genotoxic impurities from chemical reagents, chemical synthesis, and reaction are involved in all aspects of the drug synthesis process and the subsequent stability and possible degradation of the drug, which is a complex process. For potential carcinogenic or mutagenic substances and impurities with warning structures, the Ames test can generally be used to test the mutagenic ability of impurities. The Ames test is short-term in vitro test method for preliminary screening of the mutagenicity of chemical substances by using the properties of the reverse mutation of the histidine auxot...