Shanghai Medicilon Inc.

  Western blotting analysis , also known as immunoblotting or protein blotting, is a core analysis technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells. Western blots are in wide use across a broad range of scientific and clinical disciplines. Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein expression in cells, and for monitoring fractions during  protein purification . Western Blotting Analysis After the unbound probes are washed away, the western blot is ready for detection of the probes that are labeled and bound to the protein of interest. In practical terms, not all westerns reveal protein are only at one band in a membrane. Size approximations are taken by comparing the stained bands to that of the marker or ladder loaded during electrophoresi...

Medicilon Protein Separation and Purification Technology Service Sample preparation: According to customer requirements, help customers get more than 98% of samples; Preparation process design: According to customer requirements, provide protein separation and  purification services  from small trials to large-scale  production , and customize suitable processes and systems to customers according to process requirements and product characteristics; Protein separation service: According to customer requirements, to undertake the  purification  and separation of compounds from mg to kg; Impurity enrichment/separation: assist customers to separate trace and trace impurities in APIs or other compounds, cooperate with Shanghai Zhangjiang Pharmaceutical Valley Public Service Platform to provide a full set of compound separation and structure identification reports Common methods of protein separation and purification Precipitation: salting-out method, organic solv...