Shanghai Medicilon Inc.

  A   western blot   is a method in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. The proteins are then transferred out of the gel and onto a membrane, where they are “probed” using antibodies specific to the protein. As a result, researchers can examine the amount of protein in a given sample and compare levels between several groups. The Biology Department of Medicilon has extensive experience in the field of in vitro biology, through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout, RNAi and MicroRNA Technology, etc., provide a complete set of biological services. Western Blot Related Antibodies Secondary Antibody Tag Antibody Loading Control Antibody Isotype Control Antibody Techniques: Immunological Detection of Antigens Ha...

  Western blotting analysis , also known as immunoblotting or protein blotting, is a core analysis technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells. Western blots are in wide use across a broad range of scientific and clinical disciplines. Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein expression in cells, and for monitoring fractions during  protein purification . Western Blotting Analysis After the unbound probes are washed away, the western blot is ready for detection of the probes that are labeled and bound to the protein of interest. In practical terms, not all westerns reveal protein are only at one band in a membrane. Size approximations are taken by comparing the stained bands to that of the marker or ladder loaded during electrophoresi...