Protein Separation and Purification Technology Service Company

Medicilon Protein Separation and Purification Technology Service

Sample preparation: According to customer requirements, help customers get more than 98% of samples;

Preparation process design: According to customer requirements, provide protein separation and purification services from small trials to large-scale production, and customize suitable processes and systems to customers according to process requirements and product characteristics;
Protein separation service: According to customer requirements, to undertake the purification and separation of compounds from mg to kg;
Impurity enrichment/separation: assist customers to separate trace and trace impurities in APIs or other compounds, cooperate with Shanghai Zhangjiang Pharmaceutical Valley Public Service Platform to provide a full set of compound separation and structure identification reports

Common methods of protein separation and purification

Precipitation: salting-out method, organic solvent precipitation method, protein precipitation agent, polyethylene glycol precipitation, selective precipitation method
Electrophoresis: The protein is charged in the solution above or below its isoelectric point, and can move to the positive or negative electrode of the electric field in the electric field. Depending on the support, there are thin film electrophoresis, gel electrophoresis, etc.
Dialysis: A method of separating large molecular proteins from small molecular compounds using dialysis bags.
Chromatography: a. Ion exchange chromatography, which utilizes the amphoteric free nature of proteins. At a certain pH, the charge and properties of each protein are different, so it can be separated by ion exchange chromatography. Like anion exchange chromatography, proteins with a small amount of negative charge are eluted first. b. Molecular sieve, also known as gel filtration. Small molecular proteins enter the pores for a long time, and large molecular proteins cannot enter the pores and flow out.
Ultracentrifugation: It can be used to separate and purify proteins or to determine the molecular weight of proteins. Different proteins have different densities and morphologies and are separated.

Scope of protein separation and purification services

Separation, purification and concentration of chemical substances;
Concentration and desalination of dyes and dye intermediates
Product recovery during the production of ultrafine powder;
Purification and reuse of useful substances in production wastewater;
Concentration and purification of marine biological extracts;
Concentration and purification of amino acids and proteins.

Protein separation and purification

Contact us

Email: marketing@medicilon.com

Phone:021 58591500

Website: www.medicilon.com

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Protein separation and purification technology service company

Medicilon Affinity Purification Technology Service

What is preclinical testing?

In the process of preclinical testing of a compound or biological agent into a drug, the compound involved must go through the testing phase. First, we need to identify potential targets that can treat the disease. Then, a variety of compounds or preparations are screened out. Any compound that has shown potential as a drug for the treatment of this disease needs to be tested for toxicity before clinical testing to reduce the possibility of injury. preclinical testing What is the basis of preclinical testing? According to US Food and Drug Administration (FDA) regulations, a series of tests are required before a new drug is approved for use. In the first stage, basic research determines a hypothetical target for the treatment of a certain disease, and then screens small molecules or biological compounds to discover any substance with the potential to treat the disease. Then, a preclinical research phase followed, before which, as described above, the potential toxicity of the compou

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Inventory of the three major in vitro pharmacokinetic research methods

The metabolic properties of a compound are an essential factor in whether or not it can be used as a drug in the clinical setting, so pharmacokinetic studies of newly synthesized compounds are required in drug development. In vitro incubation with liver microsomes, recombinant CYP450 enzyme lines, and in vitro incubation with hepatocytes are some of the more common in vitro drug metabolism methods. 1. In vitro incubation method with liver microsomes The metabolic stability and metabolic phenotypes of candidate compounds in different species of liver microsomes are good predictors of the metabolic properties of compounds in vivo. They are practical tools for evaluating candidate compounds in the pre-development phase of drug development. Liver microsomes include rat liver microsomes, human liver microsomes, canine liver microsomes, monkey liver microsomes, and mouse liver microsomes. In in vitro incubation of the liver, microsomes are the "gold standard" for in vitro d

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Novel Parkinson’s Therapies Possible with New Mouse Model

Parkinson's disease (PD) is a neurodegenerative disorder that is marked by the accumulation of the protein, α-synuclein (αS), into clumps known as Lewy bodies, which diminish neural health. Now, researchers from Brigham and Women's Hospital (BWH) report the development of a mouse model to induce PD-like αS aggregation, leading to resting tremor and abnormal movement control. The mouse responds to L-DOPA, similarly to patients with PD. The team's study (“Abrogating Native α-Synuclein Tetramers in Mice Causes a L-DOPA-Responsive Motor Syndrome Closely Resembling Parkinson’s Disease”) on the use of this transgenic mouse model appears in Neuron . “α-Synuclein (αS) regulates vesicle exocytosis but forms insoluble deposits in PD. Developing disease-modifying therapies requires animal models that reproduce cardinal features of PD. We recently described a previously unrecognized physiological form of αS, α-helical tetramers, and showed that familial PD-causing missense mutati

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