Shanghai Medicilon Inc.

  Overview of Protein Expression System In 1983, two sample groups expressed eukaryotic genes in the colon (Harris et al., 1983; Wetzel and Goeddel, 1983). Its genetics, biochemistry and molecular biology have been well understood, so it is the preferred system for the expression of many foreign proteins. The biggest feature of the Escherichia coli expression system is low culture cost, strong tolerance, and high efficiency. The advantage of the Bacillus subtilis expression system is that the target protein can be secreted into the culture medium in a soluble active form with high yield, but it is expressed in the protein During the process, Bacillus subtilis will secrete some proteins to degrade the target protein, and the expression level of this system is often lower than that of the antibiotic expression system. Currently commonly used eukaryotic expression systems are:  mammalian cell expression system ,  yeast expression system  and  baculovirus expression...

  Process Analytical Science Our process analytical sciences group provides support for research, development and commercial production. At Medicilon, the importance of analytical support in pharmaceutical development and manufacturing is well understood.  We provide our clients with analytical method development and all other services required to support their regulatory needs. Services Offered Analytical Method Development, Qualification and Validation For Assay Related Compounds Water Content Chiral Analysis Residual Solvents Trace Metals Analysis IPC and Release Testing Impurity  Isolation and Structure Elucidation Reference Standard Qualification Stability Studies PGI Method Development, Validation and Testing Specification and Other Regulatory Filing Documentation Preparation We have the following key instrumentation to support our analytical activities Chromatography: UPLC, HPLC, LC-MS, GC, GC-HS Solid State Characterization: DSC, TGA, XRPD, PSD, Polarized Light Mi...

  Enzymatic assay Lance Assay Alphascreen Assay Z’-LYTE Assay Adapta Assay Kinase-Glo Assay ADP-Glo Assay Ligand Binding Assay ELISA Assay HTRF Assay Enzyme activity assays  are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Enzyme units : Amounts of enzymes can either be expressed as molar amounts, as with any other chemical, or measured in terms of activity, in enzyme units. Medicilon provides various  enzyme activity assays  for  kinases , phosphatases, proteinases, deacetylase, peptidase, esterase, and other enzymes. Our line of well-characterized immunoassays and biochemical kits ensures accurate and reproducible results. Enzyme is a  large category of bio-molecules  that catalyze various biological processes including metabolic processes, cellular signaling and regulation, cell division and apoptosis. Enzymatic reactions convert substrate molecules into chemically modifi...

In the process of  preclinical testing  of a compound or biological agent into a drug, the compound involved must go through the testing phase. First, we need to identify potential targets that can treat the disease. Then, a variety of compounds or preparations are screened out. Any compound that has shown potential as a drug for the treatment of this disease needs to be tested for toxicity before clinical testing to reduce the possibility of injury. preclinical testing What is the basis of preclinical testing? According to US Food and Drug Administration (FDA) regulations, a series of tests are required before a new drug is approved for use. In the first stage, basic research determines a hypothetical target for the treatment of a certain disease, and then screens small molecules or biological compounds to discover any substance with the potential to treat the disease. Then, a  preclinical research  phase followed, before which, as described above, the potential tox...

  Our synthetic chemistry team has broad and in-depth expertise in synthetic organic chemistry. We use independent synthetic route design for complex molecules. An AI system assists with this design process, as well as performing reaction outcome prediction, quotation, and more. Over the years, the team has developed and honed the skills to resolve even the most demanding synthesis-related challenges. We take a responsive project management approach and practice effective communication with each other and our clients. Scientific ethics are of the utmost importance in our synthetic chemistry division, and we strive to maintain the strongest IP protection and highest standards for data integrity. In addition, all of our projects follow green chemistry principles. Services High Quality Compound Synthesis from  mg  to  kg . Synthesis of Reagents, Intermediates, Building Blocks, Reference Compounds, Metabolites and Impurities. Scale-Up to non-GLP drug substances. Developm...