Shanghai Medicilon Inc.

  A   western blot   is a method in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. The proteins are then transferred out of the gel and onto a membrane, where they are “probed” using antibodies specific to the protein. As a result, researchers can examine the amount of protein in a given sample and compare levels between several groups. The Biology Department of Medicilon has extensive experience in the field of in vitro biology, through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout, RNAi and MicroRNA Technology, etc., provide a complete set of biological services. Western Blot Related Antibodies Secondary Antibody Tag Antibody Loading Control Antibody Isotype Control Antibody Techniques: Immunological Detection of Antigens Ha...

  LC/MS/MS Method Development & Validation Development, transfer, and optimization of methods for quantification of drugs in biological matrices  Quick turnaround time to speed up the  drug development process   Chemical Analytical Center of Medicilon  provides general analysis and purification services for the clients. Our experts have rich experience and proficient skills to solve problems, which are highly appreciated by clients. General  Analytical Chemistry  Services – NMR 1D、2D（H-NMR、C-NMR、P-NMR、F-NMR、HSQC、HMBC、COSY、NOESY, et al） –  LC-MS Analysis – HPLC Analysis (including ELSD) – Chiral HPLC Analysis – General LCMS Testing (ROI、LOD、Cl-、SO42-、mp、HM、Specific Rotation、Water Content, et al） LC/MS Introduction LC/MS is short for Liquid Chromatography / Mass Spectrometers. Liquid Chromatography (LC) is a fundamental separation technique in the life sciences and related fields of chemistry. Unlike gas chromatography, which is unsuitable for n...

  Principles The Enzyme-Linked ImmunoSorbent Assay, or  ELISA , is a technique used to detect the presence of a specific molecule in a given sample. The molecule to be detected could be an antibody or antigen. Basically, a small plastic well is coated with an antibody that binds to the antigen of interest (for example, a toxin). Sample is added to the well. If the toxin is present, it binds to the antibody and sticks to the well surface. The samples are rinsed to remove any unbound material. Another anti-toxin antibody is added. Then a tagged antibody is added; this one has an enzyme attached to one end. The substrate of the enzyme is then added. If the enzyme is there, the substrate gets converted to a colored product, and we can see it as a positive result. Procedure The surfaces of the wells in the plate are coated with a “capture” antibody. The sample is then added and any antigen present binds to the capture antibody. After washing the plates, only the antibody-antigen co...

  The Elisa experiment has been widely used clinically due to its high sensitivity and good specificity. However, each link in the operation has a greater impact on the detection effect of the experiment. If you are not careful, it may lead to incomplete color rendering and patterning. Wait for the result. ELISA Protocol The following step by step analysis of the factors that may affect the results of the Elisa test operation. Step 1: Specimen Selection and Preparation The most commonly used clinical specimens for ELISA determination are serum (plasma), and sometimes specimens such as saliva, cerebrospinal fluid, urine, and feces are also used for specific testing purposes. At present, the clinically used serum samples to determine the markers generally include antigens and antibodies of infectious pathogens, tumor markers, hormones, special proteins, cytokines, and therapeutic drugs. For the collection of serum samples used for hormone and therapeutic drug determination, it is nec...