跳至主要内容

Cell Viability Assay

 In vitro biological activity detection is a mandatory item for clinical application of biological products, with high requirements for method development, personnel operation stability, and laboratory compliance management. Medicilon's cell viability assay platform can provide compliant in vitro cell viability detection services that meet the application requirements. Our high-level scientific research team can assist customers in participating in new drug development and exploring drug action mechanisms, while our high-throughput screening platform can help customers conduct efficient drug screening.



Empowering new drug development
  • Wide variety
    Medicilon offers over 400 kinds of cell models, providing detection services and functional assay services for various signal pathways.
  • Various Methods
    Cell viability assaysReporter gene assaysTR-FRETFACSRadioactive assaysmRNA and protein expression level detection

  • Sample provision
    Customers only need to provide compounds of the target point to be tested, and we will arrange corresponding detection services and provide professional analysis reports (specific experimental cycle depends on the experiment content).
Test Content
Test items
Test content
Antibody-Mediated Immune Cell Functional Assay
ADCC, CDC, ADCP and ADC inernalization assay.
Cell Viability Assay
Cell proliferation, growth inhibition, cytotoxicity
Cell-based radiometric assay
Isotope-based binding, uptake and proliferation assays.(H3,P32,S35,I125,C14)
ELISA
Cytokine release, Necroptosis assay
FACS assay
T cell/B cell activation and proliferation assay, T cell killing, Binding/Blocking assay, cell cycle analysis, signaling pathway activation.
GPCR assay
GLP-1R/GCGR/GIPR/GLP-2R, HTRF/Reproter Assay/Calcium Influx
Immunoblot Analysis
Protein Phosphorylation/Degradation
In cell Western
Quantification of intracellular signaling in whole cells, IKZF1/3 degradation
Migration assay
Transwell
Reporter assay
NanoBiT, NanoBRET,luciferase, GFP, β-gal, β-lac
RT-PCR
mRNA expression quantification

评论

此博客中的热门博文

In Vitro Enzyme Activity Assay

  Kinases play extensive and important roles in signal transduction and co-ordination of complex functions. Currently, about 300 kinase inhibitors have been approved for marketing, among which receptor tyrosine kinase (RTK) inhibitors are the main ones. Medicilon provides high-quality kinase profile screening and has one-stop kinase research services from assay development, high-throughput screening to selectivity analysis. In addition, Medicilon provides many other enzyme activity screens. Wide Variety Provides 200+  in vitro  enzymatic detection methods; 100+ kinases, including CTK, RTK, AGC, CMGC and other kinase families as well as important common mutations Project Flexibility Various types of plates for screening, such as 96 and 384; Multiple flexible kinase profiles, including TK kinase family, CDK kinase family, etc., to meet different customer customization needs. Various Methods Fluorescence detection; Chemiluminescence detection; Absorbance detection LanthaScr...

Fluorescence Probes Synthesis

Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. Design and synthesis steps Fluorescent probes are tools used to detect, measure and analyze biomolecules, cells and tissues. They achieve these purposes by emitting and receiving fluorescent signals. Medicilon can complete the design and synthesis of fluorescent probes for biomolecules. Selection of target molecules and determination of target. Selection of fluorescent dyes. Design of molecular structure. Synthesize probe molecules. Testing the properties of probe molecules. Custom synthesis Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. After years of development, Medicilon's fluorescent probes and labeling platforms have accumulated rich experience...

Custom Synthesis

Medicilon has synthesized over 10,000 individual compounds from gram to kilogram quantities, and many compounds are synthesized with more than ten synthetic steps. The overall synthesis success rate is over 95%. High quality products from mg to kg scale Reagents, intermediates, building blocks, and scaffolds Preparation of API or reference standards Development of synthetic routes for impurities and metabolites   On-site analytical support Product delivered with COA Medicilon offers integrated drug discovery services -- Chemistry, Biology & Preclinical Services