Cell Viability Assay

In vitro biological activity detection is a mandatory item for clinical application of biological products, with high requirements for method development, personnel operation stability, and laboratory compliance management. Medicilon's cell viability assay platform can provide compliant in vitro cell viability detection services that meet the application requirements. Our high-level scientific research team can assist customers in participating in new drug development and exploring drug action mechanisms, while our high-throughput screening platform can help customers conduct efficient drug screening.

Empowering new drug development

Wide variety
Medicilon offers over 400 kinds of cell models, providing detection services and functional assay services for various signal pathways.
Various Methods
Cell viability assaysReporter gene assaysTR-FRETFACSRadioactive assaysmRNA and protein expression level detection
Sample provision
Customers only need to provide compounds of the target point to be tested, and we will arrange corresponding detection services and provide professional analysis reports (specific experimental cycle depends on the experiment content).

Test Content

Test items

Test content

Antibody-Mediated Immune Cell Functional Assay

ADCC, CDC, ADCP and ADC inernalization assay.

Cell Viability Assay

Cell proliferation, growth inhibition, cytotoxicity

Cell-based radiometric assay

Isotope-based binding, uptake and proliferation assays.(H3,P32,S35,I125,C14)

ELISA

Cytokine release, Necroptosis assay

FACS assay

T cell/B cell activation and proliferation assay, T cell killing, Binding/Blocking assay, cell cycle analysis, signaling pathway activation.

GPCR assay

GLP-1R/GCGR/GIPR/GLP-2R, HTRF/Reproter Assay/Calcium Influx

Immunoblot Analysis

Protein Phosphorylation/Degradation

In cell Western

Quantification of intracellular signaling in whole cells, IKZF1/3 degradation

Migration assay

Transwell

Reporter assay

NanoBiT, NanoBRET,luciferase, GFP, β-gal, β-lac

RT-PCR

mRNA expression quantification

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What is preclinical testing?

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Inventory of the three major in vitro pharmacokinetic research methods

The metabolic properties of a compound are an essential factor in whether or not it can be used as a drug in the clinical setting, so pharmacokinetic studies of newly synthesized compounds are required in drug development. In vitro incubation with liver microsomes, recombinant CYP450 enzyme lines, and in vitro incubation with hepatocytes are some of the more common in vitro drug metabolism methods. 1. In vitro incubation method with liver microsomes The metabolic stability and metabolic phenotypes of candidate compounds in different species of liver microsomes are good predictors of the metabolic properties of compounds in vivo. They are practical tools for evaluating candidate compounds in the pre-development phase of drug development. Liver microsomes include rat liver microsomes, human liver microsomes, canine liver microsomes, monkey liver microsomes, and mouse liver microsomes. In in vitro incubation of the liver, microsomes are the "gold standard" for in vitro d

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Enzyme Activity Assay Service

Enzymatic assay Lance Assay Alphascreen Assay Z’-LYTE Assay Adapta Assay Kinase-Glo Assay ADP-Glo Assay Ligand Binding Assay ELISA Assay HTRF Assay Enzyme activity assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Enzyme units : Amounts of enzymes can either be expressed as molar amounts, as with any other chemical, or measured in terms of activity, in enzyme units. Medicilon provides various enzyme activity assays for kinases , phosphatases, proteinases, deacetylase, peptidase, esterase, and other enzymes. Our line of well-characterized immunoassays and biochemical kits ensures accurate and reproducible results. Enzyme is a large category of bio-molecules that catalyze various biological processes including metabolic processes, cellular signaling and regulation, cell division and apoptosis. Enzymatic reactions convert substrate molecules into chemically modified molecules products with high sp

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