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Cell Viability Assay

 In vitro biological activity detection is a mandatory item for clinical application of biological products, with high requirements for method development, personnel operation stability, and laboratory compliance management. Medicilon's cell viability assay platform can provide compliant in vitro cell viability detection services that meet the application requirements. Our high-level scientific research team can assist customers in participating in new drug development and exploring drug action mechanisms, while our high-throughput screening platform can help customers conduct efficient drug screening.



Empowering new drug development
  • Wide variety
    Medicilon offers over 400 kinds of cell models, providing detection services and functional assay services for various signal pathways.
  • Various Methods
    Cell viability assaysReporter gene assaysTR-FRETFACSRadioactive assaysmRNA and protein expression level detection

  • Sample provision
    Customers only need to provide compounds of the target point to be tested, and we will arrange corresponding detection services and provide professional analysis reports (specific experimental cycle depends on the experiment content).
Test Content
Test items
Test content
Antibody-Mediated Immune Cell Functional Assay
ADCC, CDC, ADCP and ADC inernalization assay.
Cell Viability Assay
Cell proliferation, growth inhibition, cytotoxicity
Cell-based radiometric assay
Isotope-based binding, uptake and proliferation assays.(H3,P32,S35,I125,C14)
ELISA
Cytokine release, Necroptosis assay
FACS assay
T cell/B cell activation and proliferation assay, T cell killing, Binding/Blocking assay, cell cycle analysis, signaling pathway activation.
GPCR assay
GLP-1R/GCGR/GIPR/GLP-2R, HTRF/Reproter Assay/Calcium Influx
Immunoblot Analysis
Protein Phosphorylation/Degradation
In cell Western
Quantification of intracellular signaling in whole cells, IKZF1/3 degradation
Migration assay
Transwell
Reporter assay
NanoBiT, NanoBRET,luciferase, GFP, β-gal, β-lac
RT-PCR
mRNA expression quantification
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Custom Synthesis

Medicilon has synthesized over 10,000 individual compounds from gram to kilogram quantities, and many compounds are synthesized with more than ten synthetic steps. The overall synthesis success rate is over 95%. High quality products from mg to kg scale Reagents, intermediates, building blocks, and scaffolds Preparation of API or reference standards Development of synthetic routes for impurities and metabolites   On-site analytical support Product delivered with COA Medicilon offers integrated drug discovery services -- Chemistry, Biology & Preclinical Services
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In Vitro Enzyme Activity Assay

  Kinases play extensive and important roles in signal transduction and co-ordination of complex functions. Currently, about 300 kinase inhibitors have been approved for marketing, among which receptor tyrosine kinase (RTK) inhibitors are the main ones. Medicilon provides high-quality kinase profile screening and has one-stop kinase research services from assay development, high-throughput screening to selectivity analysis. In addition, Medicilon provides many other enzyme activity screens. Wide Variety Provides 200+  in vitro  enzymatic detection methods; 100+ kinases, including CTK, RTK, AGC, CMGC and other kinase families as well as important common mutations Project Flexibility Various types of plates for screening, such as 96 and 384; Multiple flexible kinase profiles, including TK kinase family, CDK kinase family, etc., to meet different customer customization needs. Various Methods Fluorescence detection; Chemiluminescence detection; Absorbance detection LanthaScr...
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Pharmacokinetics Services

Pharmacokinetics Services Our pharmacokinetics department offers the clients a broad spectrum of high quality of services in the areas of in vitro ADME, in vivo pharmacokinetics and bioanalysis services, ranging from small molecules to large molecules, such as protein and antibody. The animal species involved in our services are non-human primate, canine, mice, rat, rabbit and hamster. Meanwhile, non-human primate experimental platform and isotope platform for protein/antibody are certified by the Shanghai government. Preclinical Pharmacokinetics Method development and validation (GLP,Non-GLP) Bioanalysis Pharmacokinetics (Tumor bearing rodents, Bioavailability, Crossover studies, Cassette (N in 1) dosing and analysis, effects of gender on pharmacokinetics, BBB penetration ) Stability in vehicles and plasma Drug interaction studies In vitro metabolic stability CYP 450 inhibition Plasma protein binding studies Distribution studies Excretion studies Prediction an...
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