跳至主要内容

Discovery Biology Services


Our structural biology department offers services supporting structure-based drug discovery from determination of novel targets to final structures. Our platform is one of the earliest established structural biology platform in China and has been certified by the Shanghai government.

Enzymatic-Based Assay


Multiple Technology Platforms

  • Lance Assay
  • Alphascreen Assay
  • Z’-LYTE Assay
  • Adapta Assay
  • Kinase-Glo Assay
  • ADP-Glo Assay
  • Ligand Binding
  • ELISA Assay
  • HTRF Assay

Diversified Targets and Applications

  • Kinases
  • GPCR
  • Proteases
  • Transcription Factors
  • Cytokines
  • Metabolic Enzymes
  • Biomarker
  • Protein Binding
  • Protein Interacting
  • Compound screening

Cell-Based Assay

  • Cytotoxicity Assay
  • Apoptosis Assay
  • Cell Migration Assay
  • Cell Invasion Assay
  • HTRF Cell-based Assay
  • Immunostaining Assay
  • Immunofluorescence Assay (96/384 wells)
  • Reporter Gene Assay (GFP/Luc)
  • Radioactive Ligand/receptor Binding Assay
  • Cellular Uptake Assay
  • Gene Knockdown Assay
  • MicroRNA Overexpression Assay
  • MicroRNA Knockdown Assay
  • Adenovirus/Retrovirus/ Lentivirus 
 Medicilon has established over 100 different cancer cell lines for cell-based assays including:

  • Breast cancer cell line
  • Colorectal cancer line
  • Leukemia cell line
  • Liver cancer cell line
  • Kidney cancer cell line
  • Lung cancer cell line
  • Melanoma cell lines
  • Ovarian cell lines
  • Pancreas cell lines
  • Gastric cancer cell lines

Cell Biology & Biochemistry

  • Custom assay development 
  • Compound screening and profiling 
  • SAR study and medicinal chemistry support 
  • Middle-throughput screening 
  • Mechanism-of-action study and biomarker analysis

Radioactive Assay

  • Radioactive receptor binding assay 
  • Radioligand binding assay 
  • Radioactive uptake assay 
  • Cell proliferation assay ( H-TdR) 
  • Radioimmunoassay (RIA)
  • Immunoradiometric assay (IRMA)
Stable Cell Line Generation

Stable Cell Line Generation

  • Overexpression 
  • Knock-out 
  • Cell Lines – Mammalian cell lines

Stable Cell Line Generation-Overexpression/Knock-out

96-well immunoflouresence staining to screen for stable cell lines
PCR to screen for gene knock-out in HCT116 cells (95% positive clones)
M: DL2000 DNA Marker
  1. Clone 1-HA1 (921bp)
  2. Clone 1-HA-2 (1525bp)
  3. Clone 4-HA1 (921bp)
  4. Clone 4-HA-2 (1525bp)
  5. Clone 5-HA1 (921bp)
  6. Clone 5-HA-2 (1525bp)

RNAi & MicroRNA Technologies

Knocking down targeted protein expression in cells transfected with siRNA
Overexpression of microRNA candidates inducing apoptosis in HT29 cells

标签：

评论

发表评论

此博客中的热门博文

Custom Synthesis

Medicilon has synthesized over 10,000 individual compounds from gram to kilogram quantities, and many compounds are synthesized with more than ten synthetic steps. The overall synthesis success rate is over 95%. High quality products from mg to kg scale Reagents, intermediates, building blocks, and scaffolds Preparation of API or reference standards Development of synthetic routes for impurities and metabolites   On-site analytical support Product delivered with COA Medicilon offers integrated drug discovery services -- Chemistry, Biology & Preclinical Services
发表评论
阅读全文

In Vitro Enzyme Activity Assay

  Kinases play extensive and important roles in signal transduction and co-ordination of complex functions. Currently, about 300 kinase inhibitors have been approved for marketing, among which receptor tyrosine kinase (RTK) inhibitors are the main ones. Medicilon provides high-quality kinase profile screening and has one-stop kinase research services from assay development, high-throughput screening to selectivity analysis. In addition, Medicilon provides many other enzyme activity screens. Wide Variety Provides 200+  in vitro  enzymatic detection methods; 100+ kinases, including CTK, RTK, AGC, CMGC and other kinase families as well as important common mutations Project Flexibility Various types of plates for screening, such as 96 and 384; Multiple flexible kinase profiles, including TK kinase family, CDK kinase family, etc., to meet different customer customization needs. Various Methods Fluorescence detection; Chemiluminescence detection; Absorbance detection LanthaScr...
发表评论
阅读全文

Pharmacokinetics Services

Pharmacokinetics Services Our pharmacokinetics department offers the clients a broad spectrum of high quality of services in the areas of in vitro ADME, in vivo pharmacokinetics and bioanalysis services, ranging from small molecules to large molecules, such as protein and antibody. The animal species involved in our services are non-human primate, canine, mice, rat, rabbit and hamster. Meanwhile, non-human primate experimental platform and isotope platform for protein/antibody are certified by the Shanghai government. Preclinical Pharmacokinetics Method development and validation (GLP,Non-GLP) Bioanalysis Pharmacokinetics (Tumor bearing rodents, Bioavailability, Crossover studies, Cassette (N in 1) dosing and analysis, effects of gender on pharmacokinetics, BBB penetration ) Stability in vehicles and plasma Drug interaction studies In vitro metabolic stability CYP 450 inhibition Plasma protein binding studies Distribution studies Excretion studies Prediction an...
发表评论
阅读全文