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Discovery Biology Services


Our structural biology department offers services supporting structure-based drug discovery from determination of novel targets to final structures. Our platform is one of the earliest established structural biology platform in China and has been certified by the Shanghai government.

Enzymatic-Based Assay


Multiple Technology Platforms

  • Lance Assay
  • Alphascreen Assay
  • Z’-LYTE Assay
  • Adapta Assay
  • Kinase-Glo Assay
  • ADP-Glo Assay
  • Ligand Binding
  • ELISA Assay
  • HTRF Assay

Diversified Targets and Applications

  • Kinases
  • GPCR
  • Proteases
  • Transcription Factors
  • Cytokines
  • Metabolic Enzymes
  • Biomarker
  • Protein Binding
  • Protein Interacting
  • Compound screening

Cell-Based Assay

  • Cytotoxicity Assay
  • Apoptosis Assay
  • Cell Migration Assay
  • Cell Invasion Assay
  • HTRF Cell-based Assay
  • Immunostaining Assay
  • Immunofluorescence Assay (96/384 wells)
  • Reporter Gene Assay (GFP/Luc)
  • Radioactive Ligand/receptor Binding Assay
  • Cellular Uptake Assay
  • Gene Knockdown Assay
  • MicroRNA Overexpression Assay
  • MicroRNA Knockdown Assay
  • Adenovirus/Retrovirus/ Lentivirus 
 Medicilon has established over 100 different cancer cell lines for cell-based assays including:

  • Breast cancer cell line
  • Colorectal cancer line
  • Leukemia cell line
  • Liver cancer cell line
  • Kidney cancer cell line
  • Lung cancer cell line
  • Melanoma cell lines
  • Ovarian cell lines
  • Pancreas cell lines
  • Gastric cancer cell lines

Cell Biology & Biochemistry

  • Custom assay development 
  • Compound screening and profiling 
  • SAR study and medicinal chemistry support 
  • Middle-throughput screening 
  • Mechanism-of-action study and biomarker analysis

Radioactive Assay

  • Radioactive receptor binding assay 
  • Radioligand binding assay 
  • Radioactive uptake assay 
  • Cell proliferation assay ( H-TdR) 
  • Radioimmunoassay (RIA)
  • Immunoradiometric assay (IRMA)
Stable Cell Line Generation

Stable Cell Line Generation

  • Overexpression 
  • Knock-out 
  • Cell Lines – Mammalian cell lines

Stable Cell Line Generation-Overexpression/Knock-out

96-well immunoflouresence staining to screen for stable cell lines
PCR to screen for gene knock-out in HCT116 cells (95% positive clones)
M: DL2000 DNA Marker
  1. Clone 1-HA1 (921bp)
  2. Clone 1-HA-2 (1525bp)
  3. Clone 4-HA1 (921bp)
  4. Clone 4-HA-2 (1525bp)
  5. Clone 5-HA1 (921bp)
  6. Clone 5-HA-2 (1525bp)

RNAi & MicroRNA Technologies

Knocking down targeted protein expression in cells transfected with siRNA
Overexpression of microRNA candidates inducing apoptosis in HT29 cells

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