Shanghai Medicilon Inc.

  Medicilon provides a wide range of ion channel assays to quickly and accurately provide results for   drug discovery and development research   worldwide. We offer ion channel screening services on a variety of different high quality electrophysiology platforms. These services include: Compound Screening  Our screening compound system allows chemists and compound managers to quickly and easily purchase screening compounds for your HTS library. Cell Line Optimization Medicilon’s scientists have extensive experience of developing, optimizing and validating stable cell lines expressing recombinant and endogenous ion channels. Assay Development Medicilon’s scientists have over a decade of hands-on experience building and optimizing automated and manual patch clamp assays for a wide range of ion channel targets. Our scientists are able to fully customize ion channel assays at different stages of the screening cascade, from medium throughput SAR or hit finding through to...

  Protein kinases  are a group of enzymes with the basic  biochemical  function of phosphorylation of other proteins. Protein kinases represent one of the most important classes of cellular proteins. As the principle components of cell signal transduction pathways, the activity of kinases often controls the activities of “effector” proteins, which in turn affect the profiles of cellular gene expression. Kinase signaling pathways play a primary role in regulating cell proliferation, cell metabolism and cell death. Research has shown that changes in protein kinase activity within a cell can lead to several disease states, most notably cancer. With this knowledge base, scientists have been investigating the ability of drugs to block various protein kinases as a cancer therapy. In addition to their involvement in cancer, protein kinases are implicated in diabetes, inflammation and neurological development and degeneration. Protein Kinase Assay Medicilon is offering kinas...

  Hemolysis Assay The  hemolysis assay  is used to determine the hemolytic effect of a test compound. Hemolytic activity  is a requirement to be tested for any blood contacting medical device. The test is based on erythrocyte lysis induced by contact, leachables, toxins, metal ions, surface charge or any other cause of erythrocyte lysis. The current description is based on direct contact of biomaterial and an erythrocyte suspension. The method is based on release of hemoglobin, which can be measured spectrophotometrically. This method is suited to evaluate the hemocompatibility of biomaterials and medical devices according to the international standard ISO 10993-4:2002. The Hemolysis test is designed to determine the hemolytic properties of a medical device/material. This test is performed on medical devices/materials that have direct or indirect blood contact. Evaluate your  small molecule , biologic, or formulation excipients for its potential to lyse red bloo...

  Western blotting analysis , also known as immunoblotting or protein blotting, is a core analysis technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells. Western blots are in wide use across a broad range of scientific and clinical disciplines. Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein expression in cells, and for monitoring fractions during  protein purification . Western Blotting Analysis After the unbound probes are washed away, the western blot is ready for detection of the probes that are labeled and bound to the protein of interest. In practical terms, not all westerns reveal protein are only at one band in a membrane. Size approximations are taken by comparing the stained bands to that of the marker or ladder loaded during electrophoresi...

  Medicilon provide   preclinical animal studies   for clients. Animal   preclinical studies   of drug efficacy are an important resource for designing and performing clinical trials. They provide evidence of a drug’s potential clinical utility, inform the design of trials and establish the ethical basis for testing drugs in human. Several recent studies suggest that many preclinical investigations are withheld from publication. Such non-reporting likely reflects that private drug developers have little incentive to publish preclinical studies. However, it potentially deprives stakeholders of complete evidence for making risk/benefit judgments and frustrates the search for explanations when drugs fail to recapitulate the promise shown in animals. Preclinical Trial  – A laboratory test of a new drug or a new medical device, usually done on animal subjects, to see if the hoped-for treatment really works and if it is safe to test on humans. Two types of precli...