Protein Kinase Assay Services

Protein kinases are a group of enzymes with the basic biochemical function of phosphorylation of other proteins. Protein kinases represent one of the most important classes of cellular proteins. As the principle components of cell signal transduction pathways, the activity of kinases often controls the activities of “effector” proteins, which in turn affect the profiles of cellular gene expression. Kinase signaling pathways play a primary role in regulating cell proliferation, cell metabolism and cell death. Research has shown that changes in protein kinase activity within a cell can lead to several disease states, most notably cancer. With this knowledge base, scientists have been investigating the ability of drugs to block various protein kinases as a cancer therapy. In addition to their involvement in cancer, protein kinases are implicated in diabetes, inflammation and neurological development and degeneration.

Protein Kinase Assay

Medicilon is offering kinase assay and kinase screening service for our clients. Our talented scientists who have over decades of experience in kinase assay will offer unmatched expertise to assist you in kinase drug discovery. Moreover, we can also provide a wide range of options to save the time and cost of your discovery programs.

Kinase Assay Services Including:

– Biochemical Kinase Assays
– Kinase Antibodies & Immunoassays
– Screening & Profiling Services
– Cellular Kinase & Phosphatase Pathway Assays

Biochemical Assay

Technology Platforms

    – Fluorescence: Lance; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP etc
    – Luminescence: Kinase-Glo; ADP-Glo etc
    – Absorbance: UV; VI

Kinase Assay: VEGFR2 I

protein kinase assay

Protease Assay: Renin inhibition using FRET

kinase activity assay

Cellular Kinase Assays

In order to complement our existing biochemical assay platform, we have implemented cellular kinase assays. These assays allow to confirm inhibitors activity in a relevant cellular background and profile their selectivity against multiple signaling pathways.

Cellular Kinase Phosphorylation Assays

Cellular kinase phosphorylation assays are developed using AlphaScreen® Surefire® assay kits. The assays are optimized for directly measuring kinase activation following treatment of cells with activators of signaling pathways.

Cellular Tyrosine Kinase Receptor Activity Assays

We have demonstrated that the impedance-based technology can be used to monitor the activity of the main tyrosine kinase receptor families. These label-free assays allow the identification of inhibitors targeting either the ligand binding domain or the kinase domain. These assays can be developed in virtually any cancer cell lines or primary cells.

Contact Us

Email : marketing@medicilon.com
Tel : +86 021 58591500

Tips: Above is part of protein kinase assay, kinase activity assay and in vitro kinase assay. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

Medicilon’s Kinase Services

SPR: Protein Kinase Activity Detection Method

China Builds the World’s Largest Kinase Target Cell Screening Library

What is preclinical testing?

In the process of preclinical testing of a compound or biological agent into a drug, the compound involved must go through the testing phase. First, we need to identify potential targets that can treat the disease. Then, a variety of compounds or preparations are screened out. Any compound that has shown potential as a drug for the treatment of this disease needs to be tested for toxicity before clinical testing to reduce the possibility of injury. preclinical testing What is the basis of preclinical testing? According to US Food and Drug Administration (FDA) regulations, a series of tests are required before a new drug is approved for use. In the first stage, basic research determines a hypothetical target for the treatment of a certain disease, and then screens small molecules or biological compounds to discover any substance with the potential to treat the disease. Then, a preclinical research phase followed, before which, as described above, the potential toxicity of the compou

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Inventory of the three major in vitro pharmacokinetic research methods

The metabolic properties of a compound are an essential factor in whether or not it can be used as a drug in the clinical setting, so pharmacokinetic studies of newly synthesized compounds are required in drug development. In vitro incubation with liver microsomes, recombinant CYP450 enzyme lines, and in vitro incubation with hepatocytes are some of the more common in vitro drug metabolism methods. 1. In vitro incubation method with liver microsomes The metabolic stability and metabolic phenotypes of candidate compounds in different species of liver microsomes are good predictors of the metabolic properties of compounds in vivo. They are practical tools for evaluating candidate compounds in the pre-development phase of drug development. Liver microsomes include rat liver microsomes, human liver microsomes, canine liver microsomes, monkey liver microsomes, and mouse liver microsomes. In in vitro incubation of the liver, microsomes are the "gold standard" for in vitro d

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Novel Parkinson’s Therapies Possible with New Mouse Model

Parkinson's disease (PD) is a neurodegenerative disorder that is marked by the accumulation of the protein, α-synuclein (αS), into clumps known as Lewy bodies, which diminish neural health. Now, researchers from Brigham and Women's Hospital (BWH) report the development of a mouse model to induce PD-like αS aggregation, leading to resting tremor and abnormal movement control. The mouse responds to L-DOPA, similarly to patients with PD. The team's study (“Abrogating Native α-Synuclein Tetramers in Mice Causes a L-DOPA-Responsive Motor Syndrome Closely Resembling Parkinson’s Disease”) on the use of this transgenic mouse model appears in Neuron . “α-Synuclein (αS) regulates vesicle exocytosis but forms insoluble deposits in PD. Developing disease-modifying therapies requires animal models that reproduce cardinal features of PD. We recently described a previously unrecognized physiological form of αS, α-helical tetramers, and showed that familial PD-causing missense mutati

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