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Protein Kinase Assay Services

 Protein kinases are a group of enzymes with the basic biochemical function of phosphorylation of other proteins. Protein kinases represent one of the most important classes of cellular proteins. As the principle components of cell signal transduction pathways, the activity of kinases often controls the activities of “effector” proteins, which in turn affect the profiles of cellular gene expression. Kinase signaling pathways play a primary role in regulating cell proliferation, cell metabolism and cell death. Research has shown that changes in protein kinase activity within a cell can lead to several disease states, most notably cancer. With this knowledge base, scientists have been investigating the ability of drugs to block various protein kinases as a cancer therapy. In addition to their involvement in cancer, protein kinases are implicated in diabetes, inflammation and neurological development and degeneration.

Protein Kinase Assay

Medicilon is offering kinase assay and kinase screening service for our clients.  Our talented scientists who have over decades of experience in kinase assay will offer unmatched expertise to assist you in kinase drug discovery.  Moreover, we can also provide a wide range of options to save the time and cost of your discovery programs.

Kinase Assay Services Including:

– Biochemical Kinase Assays
– Kinase Antibodies & Immunoassays
– Screening & Profiling Services
– Cellular Kinase & Phosphatase Pathway Assays

Biochemical Assay

Technology Platforms

    – Fluorescence: Lance; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP etc
    – Luminescence: Kinase-Glo; ADP-Glo etc
    – Absorbance: UV; VI

 

Kinase Assay: VEGFR2 I

protein kinase assay

Protease Assay: Renin inhibition using FRET

kinase activity assay

Cellular Kinase Assays

In order to complement our existing biochemical assay platform, we have implemented cellular kinase assays. These assays allow to confirm inhibitors activity in a relevant cellular background and profile their selectivity against multiple signaling pathways.

Cellular Kinase Phosphorylation Assays

Cellular kinase phosphorylation assays are developed using AlphaScreen® Surefire® assay kits. The assays are optimized for directly measuring kinase activation following treatment of cells with activators of signaling pathways.

Cellular Tyrosine Kinase Receptor Activity Assays

We have demonstrated that the impedance-based technology can be used to monitor the activity of the main tyrosine kinase receptor families. These label-free assays allow the identification of inhibitors targeting either the ligand binding domain or the kinase domain. These assays can be developed in virtually any cancer cell lines or primary cells.

Contact Us 

Email : marketing@medicilon.com
Tel : +86 021 58591500

Tips:  Above is part of protein kinase assay, kinase activity assay and in vitro kinase assay. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

Related Articles:

Medicilon’s Kinase Services

SPR: Protein Kinase Activity Detection Method

China Builds the World’s Largest Kinase Target Cell Screening Library

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