Hybridoma Technology

Medicilon biology department has established an antibody drug development platform, and our team members have more than 10 years experience in custom antibody production. Medicilon's hybridoma R&D services can provide a variety of immunization methods (proteins, peptides, small molecules, whole cells) to meet with the client's needs.

Development Process of Hybridoma Technology Platform

Experimental Timeline

- 01
  Antigen preparation (provided by the customer or Medicilon)
- 02
  Standard immunization and serum titer testing
  6-8 weeks
- 03
  Fusion screening subclones
  4-6 weeks
- 04
  Providing antibody sequence
  2 weeks

Service Advantage

Advanced animal facilities:AAALAC certification;Multiple Immunization methods: Medicilon offers various antigen immunization schemes such as protein, peptide, VLP, whole cell and DNA for antigen preparation;High-throughput screening: FACS, ELISA workstation, Biacore affinity, etc., with high screening efficiency;Comprehensive functional testing services:The functional verification testing platform provides in-vitro pharmacodynamic testing services such as Affinity Ranking, Affinity Measurement, EC50 and IC50, and Cross-reactivity; etc.

Advanced Equipment

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What is preclinical testing?

In the process of preclinical testing of a compound or biological agent into a drug, the compound involved must go through the testing phase. First, we need to identify potential targets that can treat the disease. Then, a variety of compounds or preparations are screened out. Any compound that has shown potential as a drug for the treatment of this disease needs to be tested for toxicity before clinical testing to reduce the possibility of injury. preclinical testing What is the basis of preclinical testing? According to US Food and Drug Administration (FDA) regulations, a series of tests are required before a new drug is approved for use. In the first stage, basic research determines a hypothetical target for the treatment of a certain disease, and then screens small molecules or biological compounds to discover any substance with the potential to treat the disease. Then, a preclinical research phase followed, before which, as described above, the potential toxicity of the compou

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Inventory of the three major in vitro pharmacokinetic research methods

The metabolic properties of a compound are an essential factor in whether or not it can be used as a drug in the clinical setting, so pharmacokinetic studies of newly synthesized compounds are required in drug development. In vitro incubation with liver microsomes, recombinant CYP450 enzyme lines, and in vitro incubation with hepatocytes are some of the more common in vitro drug metabolism methods. 1. In vitro incubation method with liver microsomes The metabolic stability and metabolic phenotypes of candidate compounds in different species of liver microsomes are good predictors of the metabolic properties of compounds in vivo. They are practical tools for evaluating candidate compounds in the pre-development phase of drug development. Liver microsomes include rat liver microsomes, human liver microsomes, canine liver microsomes, monkey liver microsomes, and mouse liver microsomes. In in vitro incubation of the liver, microsomes are the "gold standard" for in vitro d

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Enzyme Activity Assay Service

Enzymatic assay Lance Assay Alphascreen Assay Z’-LYTE Assay Adapta Assay Kinase-Glo Assay ADP-Glo Assay Ligand Binding Assay ELISA Assay HTRF Assay Enzyme activity assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Enzyme units : Amounts of enzymes can either be expressed as molar amounts, as with any other chemical, or measured in terms of activity, in enzyme units. Medicilon provides various enzyme activity assays for kinases , phosphatases, proteinases, deacetylase, peptidase, esterase, and other enzymes. Our line of well-characterized immunoassays and biochemical kits ensures accurate and reproducible results. Enzyme is a large category of bio-molecules that catalyze various biological processes including metabolic processes, cellular signaling and regulation, cell division and apoptosis. Enzymatic reactions convert substrate molecules into chemically modified molecules products with high sp

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