Single B Cell antibody discovery technology

Medicilon can provide Single B Cell antibody discovery service, which contains Single B Cell Sorting, Single B cell Ig Gene Transcription, Amplification, and Sequencing. The principle of single B cell antibody discovery technology is that each B cell contains only a pair of functional heavy chain and light chain and each B cell has the characteristic of producing only one specific antibody.

Monoclonal antibodies (mAbs) are important tools for many diagnostic applications and research. In addition, monoclonal antibodies have become an ideal therapeutic product for many diseases, and have been well used in the treatment of cancer, autoimmune diseases and infectious diseases. A number of techniques are used to generate monoclonal antibodies for research and therapeutic purposes. Single B cell screening is a newly developed technique for rapid preparation of mab in recent years. The principle is that each B cell only contains a pair of functional heavy and light chains, and each B cell only produces aspecific antibody characteristic, which can be directly amplified from a single B cell to obtain mab. This method has the advantages of fast speed, high throughput, and natural pairing of variable regions of antibody weight and light chains. It is one of the new and efficient methods for antibody discovery.

Process of Single B Cell Antibody Production
The Concept of Single B Cell Antibody discovery technology
The principle of single B cell antibody discovery technology is that each B cell contains only a pair of functional heavy chain and light chain and each B cell has the characteristic of producing only one specific antibody. Therefore, the antibody coding sequence can be amplified directly from a single B cell to obtain a monoclonal antibody.
Single B Cell Sorting
Depending on the research application, single B cells can be isolated randomly or antigen-selectively from peripheral blood or lymphoid tissues (bone marrow, spleen). For the isolation of B cells, current single B cell sorting technology include flow cytometry, magnetic bead cell sorting, micromanipulation, laser microdissection, and microfluidic sorting. Fluorescence activated Cell Sorting (FACS) is a mature and effective single cell sorting technology.
Single B cell Ig Gene Transcription, Amplification, and Sequencing
Construction of complementary DNA (cDNA) from a single B cell provides an efficient method for simultaneous analysis of expressed IgH and IgL genes. Typically, single B-cell cDNA synthesis is performed in equipment used for cell deposition and cell lysis (96-well plates, nanowell chips, etc.), which ensures easily handling of large numbers of samples and minimizes the risk of cross-contamination. Full-length Ig variable region gene transcripts were amplified by nested PCR, and RT-PCR products were used as samples for the first round of PCR for further reactions.
Regardless of the Ig variable region gene amplification strategy used, the transcriptional information of the Ig variable region genes encoding antibody-specific single B-cells was subsequently sequenced. Then using various databases such as NCBI's IgBLAST and IMGT, the rearranged V, D and J gene segments can be easily identified and analyzed for mutations, insertions and deletions.
Case Study: Nectin-4 Mouse Serum Titer
Case Study: Nectin-4 (4-1) Sorting Single B Cell
Case Study: Nectin-4 (4-1) Sequencing (Top20)
Case Study: Nectin-4 (4-6) Sorting Single B Cell
Case Study: Nectin-4 (4-6) Sequencing (Top20)

In Vitro Enzyme Activity Assay

Kinases play extensive and important roles in signal transduction and co-ordination of complex functions. Currently, about 300 kinase inhibitors have been approved for marketing, among which receptor tyrosine kinase (RTK) inhibitors are the main ones. Medicilon provides high-quality kinase profile screening and has one-stop kinase research services from assay development, high-throughput screening to selectivity analysis. In addition, Medicilon provides many other enzyme activity screens. Wide Variety Provides 200+ in vitro enzymatic detection methods; 100+ kinases, including CTK, RTK, AGC, CMGC and other kinase families as well as important common mutations Project Flexibility Various types of plates for screening, such as 96 and 384; Multiple flexible kinase profiles, including TK kinase family, CDK kinase family, etc., to meet different customer customization needs. Various Methods Fluorescence detection; Chemiluminescence detection; Absorbance detection LanthaScr...

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Fluorescence Probes Synthesis

Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. Design and synthesis steps Fluorescent probes are tools used to detect, measure and analyze biomolecules, cells and tissues. They achieve these purposes by emitting and receiving fluorescent signals. Medicilon can complete the design and synthesis of fluorescent probes for biomolecules. Selection of target molecules and determination of target. Selection of fluorescent dyes. Design of molecular structure. Synthesize probe molecules. Testing the properties of probe molecules. Custom synthesis Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. After years of development, Medicilon's fluorescent probes and labeling platforms have accumulated rich experience...

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Custom Synthesis

Medicilon has synthesized over 10,000 individual compounds from gram to kilogram quantities, and many compounds are synthesized with more than ten synthetic steps. The overall synthesis success rate is over 95%. High quality products from mg to kg scale Reagents, intermediates, building blocks, and scaffolds Preparation of API or reference standards Development of synthetic routes for impurities and metabolites On-site analytical support Product delivered with COA Medicilon offers integrated drug discovery services -- Chemistry, Biology & Preclinical Services

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