Metabolite identification is an important
research direction in the field of biomedicine. Through the detection and
analysis of metabolites in organisms, we can understand the metabolic status of
organisms and the occurrence and development process of diseases, and provide
strong support for disease diagnosis and treatment. The MetID team of Medicilon
is composed of experienced scientists. We provide fast and reliable in
vivo and in vitro MetID and reactive metabolite capture services. We also
support new drug screening and domestic and oversees IND filings. Since the
establishment of MetID team, Medicilon has successfully completed multiple
different types of research projects for clients, including challenging peptide
MetID research.
The following are some common methods
for metabolite identification:
1. High performance liquid
chromatography (HPLC): It can separate and detect
small molecule metabolites in biological samples, and is often used for the
detection of metabolites such as amino acids, organic acids, and fatty acids.
2. Mass spectrometry (MS): It can identify metabolites with larger molecular weights in
biological samples, such as peptides, proteins, nucleic acids, etc., and is
often used in proteomics and metabolomics research.
3. Nuclear Magnetic Resonance (NMR): It can analyze the structure of organic molecules in biological
samples and is often used for the structural identification of metabolites and
the study of metabolic processes.
4. Gene knockout/transgenic technology: Through gene knockout or transgenic technology, the impact of
specific genes on the synthesis and decomposition process of metabolites is
studied.
5. Isotope labeling technology: By injecting isotope-labeled substances into organisms, the
synthesis and decomposition process of metabolites in organisms can be studied.
6. Bioinformatics methods: Through bioinformatics methods, a large amount of genome and
metabolome data are integrated and analyzed to understand the synthesis and
decomposition processes of metabolites in organisms and the functions of
related genes.
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