Metabolite identification methods

Metabolite identification is an important research direction in the field of biomedicine. Through the detection and analysis of metabolites in organisms, we can understand the metabolic status of organisms and the occurrence and development process of diseases, and provide strong support for disease diagnosis and treatment. The MetID team of Medicilon is composed of experienced scientists. We provide fast and reliable in vivo and in vitro MetID and reactive metabolite capture services. We also support new drug screening and domestic and oversees IND filings. Since the establishment of MetID team, Medicilon has successfully completed multiple different types of research projects for clients, including challenging peptide MetID research.

The following are some common methods for metabolite identification:

1. High performance liquid chromatography (HPLC): It can separate and detect small molecule metabolites in biological samples, and is often used for the detection of metabolites such as amino acids, organic acids, and fatty acids.

2. Mass spectrometry (MS): It can identify metabolites with larger molecular weights in biological samples, such as peptides, proteins, nucleic acids, etc., and is often used in proteomics and metabolomics research.

3. Nuclear Magnetic Resonance (NMR): It can analyze the structure of organic molecules in biological samples and is often used for the structural identification of metabolites and the study of metabolic processes.

4. Gene knockout/transgenic technology: Through gene knockout or transgenic technology, the impact of specific genes on the synthesis and decomposition process of metabolites is studied.

5. Isotope labeling technology: By injecting isotope-labeled substances into organisms, the synthesis and decomposition process of metabolites in organisms can be studied.

6. Bioinformatics methods: Through bioinformatics methods, a large amount of genome and metabolome data are integrated and analyzed to understand the synthesis and decomposition processes of metabolites in organisms and the functions of related genes.