跳至主要内容

A New Tool for Identifying Active Metabolites in Biological Systems

Active metabolomics is swiftly becoming a revolutionary tool in the realm of scientific research, providing us an in-depth perspective to the world of active metabolites in biological systems. Through its multi-disciplinary approach, active metabolomics, with a foundation in biochemistry, molecular biology, and computational biology, offers insights into the intricate networks of metabolites at play within biological organisms.

Active metabolites form the functional backbone within biological systems. They range from basic building blocks such as glucose and amino acids to signaling molecules such as hormones, neurotransmitters, and other biologically active entities. The identification and understanding of these metabolites are paramount for a comprehensive understanding of physiological functions in living organisms and unraveling complex biological processes.

Active metabolomics provides us with a breakthrough, allowing a systematic approach to studying active metabolites within organisms. From sampling to sample preparation, purification, qualitative and quantitative analysis using methodologies such as mass spectrometry or nuclear magnetic resonance (NMR), followed by data processing and bioinformatics analysis, active metabolomics enables a holistic understanding of metabolites and their intricate interplays.

In practice, active metabolomics requires the extraction and separation of metabolites from biological samples, followed by an analytical process using methods such as mass spectrometry, gas chromatography, or liquid chromatography. The generated data is then interpreted using computer-assisted data processing techniques, allowing for the identification of specific active metabolites, understanding their molecular structures, and exploring their metabolic pathways and mechanistic roles within biological systems.


In conclusion, the advent of active metabolomics not only helps elucidate the complex happenings within biological systems but also provides us with new perspectives and tools for the study of complex life phenomena. The deployment of this technique will undoubtedly furnish us with abundant and powerful means to delve into basic life science questions and addresses practical issues in medical health in the future.

The MetID team of Medicilon is composed of experienced scientists. We provide fast and reliable in vivo and in vitro MetID and reactive metabolite capture services. We also support new drug screening and domestic and oversees IND filings. Since the establishment of MetID team, Medicilon has successfully completed multiple different types of research projects for clients, including challenging peptide MetID research.
标签：

评论

发表评论

此博客中的热门博文

What is preclinical testing?

In the process of  preclinical testing  of a compound or biological agent into a drug, the compound involved must go through the testing phase. First, we need to identify potential targets that can treat the disease. Then, a variety of compounds or preparations are screened out. Any compound that has shown potential as a drug for the treatment of this disease needs to be tested for toxicity before clinical testing to reduce the possibility of injury. preclinical testing What is the basis of preclinical testing? According to US Food and Drug Administration (FDA) regulations, a series of tests are required before a new drug is approved for use. In the first stage, basic research determines a hypothetical target for the treatment of a certain disease, and then screens small molecules or biological compounds to discover any substance with the potential to treat the disease. Then, a  preclinical research  phase followed, before which, as described above, the potential toxicity of the compou
发表评论
阅读全文

Inventory of the three major in vitro pharmacokinetic research methods

  The metabolic properties of a compound are an essential factor in whether or not it can be used as a drug in the clinical setting, so pharmacokinetic studies of newly synthesized compounds are required in drug development. In vitro incubation with liver microsomes, recombinant CYP450 enzyme lines, and in vitro incubation with hepatocytes are some of the more common in vitro drug metabolism methods. 1. In vitro incubation method with liver microsomes The metabolic stability and metabolic phenotypes of candidate compounds in different species of liver microsomes are good predictors of the metabolic properties of compounds in vivo. They are practical tools for evaluating candidate compounds in the pre-development phase of drug development. Liver microsomes include rat liver microsomes, human liver microsomes, canine liver microsomes, monkey liver microsomes, and mouse liver microsomes. In in vitro incubation of the liver, microsomes are the "gold standard" for in vitro d
发表评论
阅读全文

Novel Parkinson’s Therapies Possible with New Mouse Model

Parkinson's disease (PD) is a neurodegenerative disorder that is marked by the accumulation of the protein, α-synuclein (αS), into clumps known as Lewy bodies, which diminish neural health. Now, researchers from Brigham and Women's Hospital (BWH) report the development of a mouse model to induce PD-like αS aggregation, leading to resting tremor and abnormal movement control. The mouse responds to L-DOPA, similarly to patients with PD. The team's study (“Abrogating Native α-Synuclein Tetramers in Mice Causes a L-DOPA-Responsive Motor Syndrome Closely Resembling Parkinson’s Disease”) on the use of this transgenic mouse model appears in  Neuron . “α-Synuclein (αS) regulates vesicle exocytosis but forms insoluble deposits in PD. Developing disease-modifying therapies requires animal models that reproduce cardinal features of PD. We recently described a previously unrecognized physiological form of αS, α-helical tetramers, and showed that familial PD-causing missense mutati
发表评论
阅读全文