Gene Knockout Technology Service Company

The Medicilon gene knockout service has been successfully applied to model organisms such as cells, yeast, plants, zebrafish, mice, and rats. Gene knock-out is a technique for the targeted integration of foreign genes into a certain location on the genome of target cells through homologous recombination to achieve the purpose of targeted modification of a gene on a chromosome. It overcomes the blindness and chance of random integration, and is an ideal method to modify and transform biological genetic material.

Gene knockout service

1. CRISPR/Cas9 gene knockout mouse technical service

Technical process: gRNA vector construction =>”gRNA and Cas9 transcribed into mRNA in vitro => “mRNA microinjection (produced by F0 model)

Provide the following technical services:

All-inclusive services from gRNA vector construction to F0 model production

Customers provide Cas9 RNA and gRNA that meet quality requirements for subsequent microinjection and model production

2. Traditional ES target gene knockout/knock-in mouse technical service

Technical process: Targeting vector design and construction => “ES electrotransfer and positive clone screening => “ES microinjection and chimera preparation =>”F1 generation mouse reproduction and identification

Provide the following technical services: complete gene knockout mice, conditional gene knockout mice, gene knock-in mice

What is a knockout mice model?

After knocking out one or more of all the 30,000 genes in the mouse, the cultured mouse becomes an animal model suitable for drug clinical trials, which can imitate human patients for research and treatment. For example, there is a key link in pharmaceutical research and development, which is animal experiments. In order to study drugs for treating cancer, a mouse model of cancer is required; in order to study diabetes, a mouse model of diabetes is required.

How do you make a knockout mouse?

1.Obtaining ES cells
2.Construction of gene vector
3.Introduction of the target gene
4.Screen the hit cells with selective medium
5.By observing the changes in the biological shape of the chimeric mice and then understanding the changes in the biological shape of the mice before and after the change of the target gene, the purpose of studying the target gene is achieved.

Application areas of gene knockout

1.Establish a transgenic animal model of human diseases to provide materials for medical esearch
2.Transform animal genotypes, identify new genes and/or their new functions, and study developmental biology
3.Treatment of genetic diseases
4.Transform organisms and cultivate new biological varieties

Knockout Gene Model Has a Nose for Knockout Breath

Distract DNA Repair to Cinch CRISPR Knockout

In Vitro Enzyme Activity Assay

Kinases play extensive and important roles in signal transduction and co-ordination of complex functions. Currently, about 300 kinase inhibitors have been approved for marketing, among which receptor tyrosine kinase (RTK) inhibitors are the main ones. Medicilon provides high-quality kinase profile screening and has one-stop kinase research services from assay development, high-throughput screening to selectivity analysis. In addition, Medicilon provides many other enzyme activity screens. Wide Variety Provides 200+ in vitro enzymatic detection methods; 100+ kinases, including CTK, RTK, AGC, CMGC and other kinase families as well as important common mutations Project Flexibility Various types of plates for screening, such as 96 and 384; Multiple flexible kinase profiles, including TK kinase family, CDK kinase family, etc., to meet different customer customization needs. Various Methods Fluorescence detection; Chemiluminescence detection; Absorbance detection LanthaScr...

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Fluorescence Probes Synthesis

Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. Design and synthesis steps Fluorescent probes are tools used to detect, measure and analyze biomolecules, cells and tissues. They achieve these purposes by emitting and receiving fluorescent signals. Medicilon can complete the design and synthesis of fluorescent probes for biomolecules. Selection of target molecules and determination of target. Selection of fluorescent dyes. Design of molecular structure. Synthesize probe molecules. Testing the properties of probe molecules. Custom synthesis Medicilon's fluorescent probes and labeling platforms can provide fluorescent probe design and synthesis services for scientific institutions, drug R&D companies, and other customers. After years of development, Medicilon's fluorescent probes and labeling platforms have accumulated rich experience...

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Custom Synthesis

Medicilon has synthesized over 10,000 individual compounds from gram to kilogram quantities, and many compounds are synthesized with more than ten synthetic steps. The overall synthesis success rate is over 95%. High quality products from mg to kg scale Reagents, intermediates, building blocks, and scaffolds Preparation of API or reference standards Development of synthetic routes for impurities and metabolites On-site analytical support Product delivered with COA Medicilon offers integrated drug discovery services -- Chemistry, Biology & Preclinical Services

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